TEST NAME
– FISH t(9;22) BCR/ABL
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ABOUT TEST
– Philadelphia (Ph) chromosome that results from a reciprocal t(9;22)(q34;q11.2) translocation or its variants t(V;9;22). The molecular consequence of the t(9;22)(q34;q11.2) translocation is the formation of the BCR/ABL fusion gene, which is usually located on the Ph chromosome. Fluorescence in situ hybridization (FISH) is used to confirm the presence of a BCR-ABL1 gene in the initial diagnosis of CML or Ph-positive ALL or AML. FISH has the advantage that it may detect cryptic BCR-ABL1 rearrangements not picked up by karyotyping and also possible deletions in the derivative 9 (der (9)) chromosome. FISH is also a valuable tool in determining the percentage patient’s blood or bone marrow cells harboring the Ph chromosome and to monitor response to treatment and disease recurrence. In addition, Dual color Dual Fusion FISH probe is the most sensitive probe in detecting t(9;22)(q34;q11.2) translocation.
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GENES TESTED
– t(9;22) (q34; q11.2)
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SAMPLE REQUIREMENTS
– Green
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TAT
– 10
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Related Tests
– JAK2 V617V mutation and BCR/ABL by PCR (Major breakpoint)
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